Human cot1 dna的作用
WebLichter P, Cremer T, Borden J et al (1988) Delineation of individual human chromosomes in metaphase and interphase cells by in situ suppression hybridization using recombinant DNA libraries. Hum Genet 80:224–234 Britten RJ, Graham DE, Neufeld BR (1974) Analysis of repeating DNA sequences by reassociation. In: Methods in Enzymology. WebHuman Cot-1 DNA Reactivity 0 Special Hazard 0 Form: M013M-003 Effective Date: 01/30/2024 Section 1: Chemical Product and Company Identification Product Name: Human Cot-1 DNA Catalog Codes: MPNA-8888, 8889, C150, C650, CUS1, CUS2, 10MG Identified Uses: Analytical reagent Brand: Molecular Biologicals International, 1Inc. Contact …
Human cot1 dna的作用
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WebHuman Cot-1 DNA® Cat. No. Size: 15279-011 500 µg 15279-101 1 mg Conc: 1 mg/ml Store at -20°C Description Human Cot-1 DNA® is obtained from human placental DNA by extracting, shearing, denaturing, and reannealing DNA under conditions that enrich for repetitive DNA sequences such as the Alu I and Kpn I families (1,2). WebBackground hybridization due to repetitive DNA sequences is a common problem in microarray assays. This cross-hybridization makes it difficult to identify differentially expressed genes, especially lower-abundant messages. Cot-1 DNA® blocking reagent blocks repetitive DNA sequences and prevents nonspecific hybridization, providing you …
WebHuman Cot-1 DNA is commonly used to block nonspecific hybridization in microarray screening. It can also be used to suppress repetitive DNA sequences for the direct mapping of human DNA or mapping genomic … Web在微阵列分析、滤光片和荧光原位杂交实验中，Human Cot-1 DNA 用于阻断非特异性杂交；还能用于抑制重复 DNA 序 列、以便直接对人 DNA 进行定位或将基因组克隆定位至体 …
WebHuman Cot-1 DNA™ is commonly used to block nonspecific hybridization in microarray screening. It can also be used to suppress repetitive DNA sequences for the direct … WebCOT Human DNA (A 260, water): 1.0-2.0 mg/mL Y-Chromosom (recovered exclusively from male human placenta): Corresponds to specification A 260 /A 280: 1.6-2.0 Absence of HIV 1/2 and HCV/HBV: Corresponds to specification Performance test using gel electrophoretic separation (4% agarose gel, without RE cleavage): middle chain length: 50-300 bp
WebThe Cot-1 Human DNA product is optimized for CGH (comparative genomic hybridization) and CGH+SNP (single nucleotide polymorphism) microarray workflows. The product is …
WebCOT Human DNA can be used to suppress nonspecific hybridization to human repetitive sequences in microarray analysis, and in filter and in situ hybridization experiments.", … grove point financial newsWeb21 mrt. 2024 · Human cot1 DNA：主要用于封阻基因组中一些高度重复的序列 Universal block oligo：主要用于封阻接头序列（与接头序列反向互补） 鲑鱼精DNA：保护cot1 … filmora per windows 10WebHuman Cot-1 DNA™ is commonly used to block nonspecific hybridization in microarray screening. It can also be used to suppress repetitive DNA sequences for the direct … filmora photoshopgrove point marinas ownersWebProduct Details. Liquid. In 10 mM TrisHCl, pH 7.5, containing 1 mM EDTA. Human Cot DNA can be used to block repetitive DNA sequences in a variety of genomics applications. Repetitive sequences of DNA are found throughout the human genome. These repetitive DNA elements often present difficulties in acquiring clean results from hybridization ... grove police officeWebQuantifying Cot-1 DNA™ by fluorometry provides more accurate concentration data of higher molecular weight products, making it beneficial to certain genomic-based array … grove point financial reviewsWebHere we present two protocols: the first one describes rapid COT DNA isolation and the peculiarities of its use in different FISH experiments, and the second is used for COT-free FISH with complex probes and is based on a special software tool for image enhancement. less Based on techniques References grove pony club