Hoechst nuclear dye

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August undefined, 2024

Nettet1. jan. 2024 · Few of the most popularly used fluorescent dyes for nuclear staining include DAPI, DIPI, Hoechst 33258, 33342. Hoechst stain 33342 is reported to be more popular for its greater cell membrane permeability in comparison with Hoechst stain 33258 and at the same time four times costlier. NettetHoechst 34580 Synonym (s): N,N-Dimethyl-4- [5- (4-methyl-1-piperazinyl) [2,5′-bi-1H-benzimidazol]-2′-yl]benzenamine trihydrochloride, HOE 34580 Empirical Formula (Hill Notation): C27H29N7 · 3HCl CAS No.: 911004-45-0 Molecular Weight: 560.95 Match Criteria: Product Name, Keyword All Photos ( 1) HOECHST 2495 Linear Formula: …

Hoechst - Biotium

NettetFar red nuclear staining dye with greater photostability than traditional blue fluorescent nuclear stains such ... Pricing; SCT119: Live cell imaging red nuclear staining dye with greater photostability than traditional blue fluorescent nuclear stains such as DAPI and Hoechst 33342. Expand. Hide. Match Criteria: Product Name. All Photos (1 ... NettetFeatures of Hoechst 33342 Fluorescent Stain: • Hoechst dye—blue fluorescent stain specific for DNA (i.e., nuclei of eukaryotic cells) • Convenient—provided as an easy-to … Thermo Scientific Pierce Hoechst 33342 Fluorescent Stain is a high-quality … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes While most cells have a single nucleus, some cell types do not have a nucleus … The Thermo Scientific FITC Antibody Labeling Kit is a multi-component kit … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes high speed automatic folder gluer

Hoechst or DAPI for nuclear staining? - Biology Stack Exchange

NettetHoechst 33258 Staining Dye Solution (ab228550) is a fluorescent stain for labeling DNA in fluorescence microscopy. This product may be used in fluorescence microscopy, microplate, cuvette and flow cytometry applications. It can also be used to detect the contents of a sample DNA by plotting a standard emission-to-content curve. Nettet14. aug. 2024 · Not all tested DNA dyes did reflect chromatin compaction in their lifetime (See Table S1, list of tested DNA dyes). However, similar results to Hoechst 34580 could be obtained using Syto13. The fluorescence lifetime of Syto 13 responds to VPA or 4-fold PBS treatment in a range comparable to Hoechst 34580 . NettetHoechst 33342 is a bisbenzimidazole dye with high specificity for binding to double-stranded DNA (preferentially binds to A-T base pairs). This dye is very useful to label … high speed automatic card punching machine

PureBlu Hoechst 33342 Nuclear Staining Dye - Bio-Rad …

NettetFor live detection of ROS production, L6 cells were seeded at 70% confluency on a µ-slide 4-well chambered polymer coverslip (Ibidi), and pre-stained 15 min prior to imaging with Hoechst nuclear dye (concentration) (x). The growth medium was then replaced with 0.5% FBS AMEM. NettetHoechst 33342 is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to DNA. With NucBlue™ Live ReadyProbes™ Reagent we … how many days holiday do teachers getNettetWash the counterstained cells with either PBSA or Hanks Balanced Salt Solution (depending upon the nuclear dye) for three times at 5 minutes for each wash. Protect … how many days ho chi minh city

"NettetHoechst 33342 is a well-characterized blue-emitting fluorescent compound widely utilized for nuclear staining. Hoechst 33342 permeates cell membranes and binds to the … " - Hoechst nuclear dye

Hoechst nuclear dye

NettetHoechst stains are part of a family of blue fluorescent dyes commonly used to stain DNA. It gets incorporated biosynthetically into interphase nuclei in fixed cytologic … NettetNuclear weapons testing is the act of experimentally and deliberately firing one or more nuclear devices in a controlled manner pursuant to a military, scientific or technological goal. This has been done on test sites on …

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NettetLive cell imaging green nuclear staining dye with greater photostability than traditional blue fluorescent nuclear stains such as DAPI and Hoechst 33342. SCT120 1 Kit Price could not be retrieved Recommended Products Overview Supporting Documentation Related Products & Applications Overview NettetHoechst dyes are cell membrane-permeant, minor groove-binding blue fluorescent DNA stains. These dyes are widely used in cell cycle and apoptosis studies as nuclear …

Nettet7. mai 2024 · Hoechst dyes are popular blue fluorescent, nuclear-specific dyes used to stain live or fixed cells. They bind preferentially to adenine-thymine (A-T) regions of DNA and exhibit distinct fluorescence emission spectra dependent on dye:base pair ratios. Hoechst dyes are excited by UV light (~360 nm) or mercury-arc lamps, or UV lasers … Both dyes are excited by ultraviolet light at around 350 nm, and both emit blue-cyan fluorescent light around an emission spectrum maximum at 461 nm. Unbound dye has its maximum fluorescence emission in the 510–540 nm range. Hoechst stains can be excited with a xenon- or mercury-arc lamp or with an ultraviolet laser. There is a considerable Stokes shift between the excitation an…

NettetPerforming nuclear staining with DAPI either Hoechst? Check out we detailed print for staining live cells or fixed cells and weaves. Sales & Resources. Order Support. Ordering Information Quick Order Find adenine Distributor Contact Customer Service Request Custom Conjugation . Technical Resources. Frequently Asked Faqs ... NettetA common approach used to assess DNA repair factor binding in mammalian cells is to induce DNA damage with a UV laser and follow the movement of GFP-tagged proteins to the site of damage. Often...

Nettet2. jan. 2024 · Nuclear-ID Red DNA stain is a novel DNA-intercalating dye that freely enters live cells, allowing cell cycle analysis without laborious permeabilization and fixation steps.

NettetAAT Bioquest's selection of Hoechst stains includes Hoechst 33342, Hoechst 33258, Hoechst 34580, and Hoechst S769121 (Nuclear Yellow), supplied as solids and UltaPure grade solids. ... Like DAPI, Hoechst dyes preferentially bind to the adenine-thymine-rich sequences in the minor grooves of double-stranded DNA. high speed automatic boxing machineNettet26. nov. 2012 · The Hoechst 33342 dye is similar to DAPI in that both are UV-excited, minor groove-binding, and emit signals proportional to total DNA content. Both are maximally excited around 355 nm and emit around 460 nm. A UV light source is required, which may harm the cell. high speed automotive groupNettetFar-red nuclear counterstains for the Cy®5 channel, such as RedDot™2, also can be used to avoid this problem. Check out our Tech Tip: Avoiding Artifacts from UV Photoconversion of DAPI and Hoechst for more information on troubleshooting staining issues with DAPI and Hoechst blue nuclear dyes. Blotting membrane autofluorescence high speed automatic printing machineNettetvarious nuclear sizes and distribution were seeded at five different cell densities and the metabolic phenotypes were examined by employing the XF Cell Energy Phenotype Test. As described above, Hoechst 33342 was included in the oligomycin/FCCP mixture. The data were normalized by the cell numbers calculated by the XF Imaging and Cell Counting high speed automotive and marine repairNettet13. apr. 2024 · Receptor-interacting protein kinase 1 (RIPK1) contributes to necroptosis. Our previous study showed that pharmacological or genetic inhibition of RIPK1 protects against ischemic stroke-induced ... how many days holiday do teachers haveNettetHoechst 33342 is a bisbenzimidazole dye with high specificity for binding to double-stranded DNA (preferentially binds to A-T base pairs). This dye is very useful to label double-stranded DNA and thus to visualize nuclei. Hoechst 33342 can be excited at ~355 nm by a UV light source (eg, UV laser beam or a mercury arc-lamp). how many days holiday entitlement per yearNettetProduct overview. Hoechst 33342 Staining Dye Solution (ab228551) is a fluorescent stain for labeling DNA in fluorescence microscopy. This product may be used in fluorescence microscopy, microplate, cuvette, and flow cytometry applications. It can also be used to detect the contents of a sample DNA by plotting a standard emission … how many days holiday do americans get