Ctll-2 ed50
Web一种重组人il2包涵体纯化复性方法 技术领域 1.本技术涉及一种重组人il2包涵体纯化复性方法，属于蛋白表达纯化技术领域。 背景技术： 2.白细胞介素2(il2)又称t细胞生长因子，是由t细胞(cd4+和cd8+)和nk细胞产生，主要受抗原和t细胞受体结合刺激分泌。 il2基因位于染色体4q26-28,长度3662bp,含4个外显子和3 ... WebNational Center for Biotechnology Information
Ctll-2 ed50
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WebCTLL-2 cell proliferation: ED50 ≤ 5 ng/mL: Preparation and Storage Country of Origin: USA Shipping: Room temperature Storage Prior to Reconstitution: -20°C Product Reconstitution: Sterile 10 mM sodium bicarbonate, pH 8.5 at 0.1 mg/mL Instructions: Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended ... WebThe newly developed cell lines (termed CTLL-2_NF-kB and A20_NF-kB) were used to implement a general bioassay, which was compared to established procedures for the characterization of various types of engineered cytokine products. Moreover, the new methodologies allowed the discovery of a novel strategy for cytokine potentiation, based …
WebMeasure by its ability to induce proliferation in CTLL-2 cells. The ED50 for this effect is <0.2 ng/mL. The specific activity of recombinant human IL-2 is approximately >2.5 x 107 IU/mg. To demonstrate lot-to-lot consistency of GMP IL-2, the activity of three separate lots was examined and plotted on the same graph. WebEach lot has its own ED50 determined using an activity assay based on a specific function of the growth factor. The activity of our IL2 Recombinant Human growth factor is …
WebBioactivity: The ED50 of this protein, as determined by increased proliferation of mouse CTLL-2 cells stimulated with 1 ng/mL IL-2, is 0.5-1.5 µg/mL. Endotoxin: Less than 0.1 … WebMouse IL-2, amino acids Phe22-Gln169 (Accession# NM_008366), was expressed in E. coli . The 148 amino acid recombinant protein has a predicted molecular mass of 17.2 kD. The predicted N-terminal amino acid is Phe. >98%, as determined by Coomassie stained SDS-PAGE and HPLC analysis. Lyophilized, carrier-free.
WebCTLL-2 cells were seeded in a 96-well plate and treated with varying concentrations of Human Recombinant IL-2. Cell proliferation was measured using a colorimetric assay. In the graph below, the specific …
WebR&D Systems™ Recombinant Human IL-2 Protein. Version with mutation found in aldesleukin Available! Supplier: R&D Systems™ 202IL050. View more versions of this … images of meadow croft godalmingWebIL-15 was discovered in the supernatant from a simian kidney epithelial cell line CV-1/EBNA, as a soluble factor capable of supporting proliferation of the IL-2-dependent cell line, … list of amino acids in human bodyWebED50 = 0.1 - 0.4 ng/mL; determined by the dose-dependent proliferation of mouse CTLL-2 cells. Conjugate: Unconjugated : Form: Lyophilized: Purification: sequential chromatography: Contains: no preservative: ... Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of ... images of meaghan pinkerton maineWebED 50 =2.5 - 15 ng/ml, corresponding to a specific activity of 0.6 - 4 x 10 5 units/mg, as determined by the dose dependent stimulation of CTLL-2 cell proliferation. Application … images of measles rash in toddlerWebBackground: Interleukin 2 (IL-2) is a T cell stimulatory cytokine best known for inducing activated T cell proliferation . in vitro ... Bioactivity: ED50 is determined by the dose-dependent Proliferation of CTLL- 2 cells. The ED50 is typically less than 3 ng/mL. The international units of CTGrade rh IL-2. images of meals on wheelsWebctll-2 Cells. Organism Type. Mouse. Cytotoxic T cell line (CD8+) Subculturing: Subculture actively growing suspension cultures before they the reached 1 X 10 exp5 cell/ml. Use … list of amish authorsWebHuman IL-2, 1 mg. $775.00. Add to Basket. Induced proliferation of CTLL-2 cells assay for Human IL-2. Cell proliferation was measured to calculate the ED50, which is as expected … images of meanders